Images were collected on a Zeiss LSM 800 completed system confocal imager. Quantification of immunostaining was done by 1 or 2 blinded investigators using the ImageJ cell counter manually. For corpus callosum imaging, one medial and two lateral images of corpus callosum and cingulum were taken from 3 or 4 sections per slide (n = 3). For motor cortex imaging, a minimum of four images were analyzed from 3 animals. For spinal cord demyelinating lesions, the lesion was located by visualizing the accumulation of Hoechst-positive nuclei within the ventral white matter. A minimum of three lesion sections from 3 mice were analyzed for cell density. For cuprizone, three regions from four sections per animal were analyzed (n = 4). Density per square millimeter was calculated in Microsoft Excel as previously described by Chamberlain et al. (2017) (link). All statistics were performed using Prism. Data are expressed as mean ± SEM. Comparisons were analyzed by two-way ANOVA with Sidak's multiple comparison test, one-way ANOVA with Tukey's multiple comparison test, or two-tailed t test. Diagrams were generated using Mind The Graph.
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