Generation of Nestin-Cre Hsd11b2 KO Mice

Hsd11b2^f/f mice were generated on a C57BL6 background (Artemis Pharmaceuticals, Cologne, Germany) by inserting LoxP sites into intron 1 and 5. These mice were bred with transgenic mice expressing Cre recombinase under the control of a rat nestin promoter/enhancer (B6.Cg-Tg(Nes-cre)1Kln/J; Jackson Laboratory, USA), as we described.^{23 (link)} This generated Nestin-Cre.Hsd11b2^fl/fl offspring (Hsd11b2 Brain Knock-out; Hsd11b2.BKO) and Hsd11b2^fl/fl littermate controls. All experiments were performed blinded to genotype and in accordance with the United Kingdom Home Office Animals (Scientific Procedures) Act, following ethical review by the University.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Evans L.C., Ivy J.R., Wyrwoll C., McNairn J.A., Menzies R.I., Christensen T.H., Al-Dujaili E.A., Kenyon C.J., Mullins J.J., Seckl J.R., Holmes M.C, & Bailey M.A. (2016). Conditional Deletion of Hsd11b2 in the Brain Causes Salt Appetite and Hypertension. Circulation, 133(14), 1360-1370.

Publication 2016

B6.Cg-Tg(Nes-cre)1Kln/J

Animals Brain Cre recombinase Ethical review Genotype Intron Mice Nestin Pharmaceuticals Transgenic mice

Corresponding Organization : University of Southern Denmark

Top 5 similar protocols

Variable analysis

independent variables

Cre recombinase expression under the control of a rat nestin promoter/enhancer

dependent variables

Hsd11b2 gene knockout in the brain

control variables

C57BL6 mouse background
Hsd11b2^fl/fl littermate controls

controls

Positive control: Hsd11b2^fl/fl littermate controls
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!