Total RNA was isolated with the RNeasy Mini Kit (QIAGEN, Hilden, Germany) and treated with RNase-Free DNase (QIAGEN). cDNA was generated from DNase-treated total RNA by using random primers (Invitrogen, Carlsbad, CA) with Superscript II reverse transcriptase (Invitrogen). The primer sequences were as follows: SST forward, 5ʹ-CCA GAC TCC GTC AGT TTC TGC A-3ʹ; SST reverse, 5ʹ-CAT CAT TCT CCG TCT GTT TGG GTT-3ʹ [10 (link)]; SSTR1 forward, 5ʹ- TCT GCG CGA AGA TCG TCA AC-3ʹ; SSTR1 reverse, 5ʹ- GCG GCT CTG GAC TGG TAA ATG-3ʹ (TaKaRa, Tokyo, Japan); GAPDH forward, 5ʹ-GCA CCG TCA AGG CTG AGA AC-3ʹ; and GAPDH reverse, 5ʹ-TGG TGA AGA CGCCAG TGG A-3ʹ. To analyze the expression of SSTR2, SSTR3, SSTR4, and SSTR5, we used previously described primers and conditions. [19 (link)] Quantitative-RT-PCR was performed on the TaKaRa TP800 system. Quantitative RT-PCR was carried out as described previously. [4 (link)]
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