Weight and height were measured and BMI calculated as weight(kg)/(height(m))2. Waist circumference was measured to the nearest 0.1 cm. Blood pressure was measured with a random zero sphygmomanometer.
Venous blood samples were drawn from the right antecubital vein after an overnight fast. For blood count analysis, whole blood was anticoagulated with EDTA. Blood cell parameters were measured by flow cytometric particle counting (cells) and photometry (Hb) using Sysmex XE- 5000 and XT-2000i analyzers (Sysmex Corporation) with reagents provided by the manufacturer (Cellpack and Sulfolyser).
For the biochemical measurements, serum was separated, aliquoted and stored at −70 °C until analysis. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GT), glucose, cholesterol, and triglyceride concentrations were measured with ALT, AST, GT, Glucose, Cholesterol, and Triglycerides System Reagent, (Beckman Coulter Biomedical). Apolipoprotein A1 (ApoA1), apolipoprotein B (ApoB), and C-reactive protein (CRP) were determined immunoturbidimetrically (ApoA1 and B assay reagent, Orion Diagnostica and CRP Latex reagent, Beckman Coulter Biomedical). The serum triglyceride concentration was assayed using the enzymatic glycerol kinase–glycerol phosphate oxidase method (Beckman Coulter Biomedical). Serum total cholesterol levels were measured by the enzymatic cholesterol esterase–cholesterol oxidase method (Beckman Coulter Biomedical). The same reagent was used for estimating HDL cholesterol levels after the precipitation of low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL) with dextran sulfate- Mg2+. Serum glucose concentrations were determined by the enzymatic hexokinase method (Beckman Coulter Biomedical). All the above-mentioned assays were performed on an AU400 instrument (AU400, Olympus).
Glycated hemoglobin (HbA1c) fraction in whole blood was measured by an Abbott Architect ci8200 analyzer (Abbott Laboratories). The concentration of total hemoglobin was first determined colorimetrically, after which the concentration of HbA1c was measured immunoturbidimetrically using the microparticle agglutination inhibition method (Fisher Diagnostics). These two concentrations were used to calculate the HbA1c percentage. All the above-mentioned methods besides ALT, AST, and GT quantifications were accredited by the Finnish Accreditation Service (FINAS).
Venous blood samples were drawn from the right antecubital vein after an overnight fast. For blood count analysis, whole blood was anticoagulated with EDTA. Blood cell parameters were measured by flow cytometric particle counting (cells) and photometry (Hb) using Sysmex XE- 5000 and XT-2000i analyzers (Sysmex Corporation) with reagents provided by the manufacturer (Cellpack and Sulfolyser).
For the biochemical measurements, serum was separated, aliquoted and stored at −70 °C until analysis. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GT), glucose, cholesterol, and triglyceride concentrations were measured with ALT, AST, GT, Glucose, Cholesterol, and Triglycerides System Reagent, (Beckman Coulter Biomedical). Apolipoprotein A1 (ApoA1), apolipoprotein B (ApoB), and C-reactive protein (CRP) were determined immunoturbidimetrically (ApoA1 and B assay reagent, Orion Diagnostica and CRP Latex reagent, Beckman Coulter Biomedical). The serum triglyceride concentration was assayed using the enzymatic glycerol kinase–glycerol phosphate oxidase method (Beckman Coulter Biomedical). Serum total cholesterol levels were measured by the enzymatic cholesterol esterase–cholesterol oxidase method (Beckman Coulter Biomedical). The same reagent was used for estimating HDL cholesterol levels after the precipitation of low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL) with dextran sulfate- Mg2+. Serum glucose concentrations were determined by the enzymatic hexokinase method (Beckman Coulter Biomedical). All the above-mentioned assays were performed on an AU400 instrument (AU400, Olympus).
Glycated hemoglobin (HbA1c) fraction in whole blood was measured by an Abbott Architect ci8200 analyzer (Abbott Laboratories). The concentration of total hemoglobin was first determined colorimetrically, after which the concentration of HbA1c was measured immunoturbidimetrically using the microparticle agglutination inhibition method (Fisher Diagnostics). These two concentrations were used to calculate the HbA1c percentage. All the above-mentioned methods besides ALT, AST, and GT quantifications were accredited by the Finnish Accreditation Service (FINAS).
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