Subcellular Fractionation of GlDRP Proteins
Corresponding Organization : University of Zurich
Variable analysis
- Expression of GlDRP-HA and GlDRP-K43E-HA transgenes
- Presence and localization of HA-tagged proteins in the soluble (supernatant) and membrane (pellet) fractions
- Centrifugation conditions (14,000 x g for 10 minutes at 4°C)
- Experimental techniques (freeze-thaw lysis, SDS-PAGE, Western blot)
- Positive control: Detection of HA-tagged proteins using a rat anti-HA mAb (clone 3F10, Roche)
- Negative control: Not explicitly mentioned
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