For sub-cellular fraction experiments, 4.106GlDRP-HA and GlDRP-K43E-HA- expressing transgenic cells were lysed by freeze-thawing and supernatant (soluble fraction) and pellet (membrane fraction) were prepared by centrifugation at 14’000 x g for 10 minutes at 4°C. The HA-tagged proteins were detected by SDS-PAGE and Western blot using a rat anti-HA mAb (clone 3F10, Roche) as described previously [54 (link)].
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