Comprehensive Flow Cytometry Analyses

Flow cytometric analyses were performed as previously described [57 (link)]. eBioscience antibodies were used unless otherwise specified: TCRβ (H57–597), CD4 (RM4–5), CD8 (53–6.7), CD19 (eBio1D3), CD45 (30-F11), CD11b (M1/70), CD44 (IM7), CD62L (MEL14), CD11c (N418), Ly6G (1A8), PDGFRα (APA5), O4 (O4; Miltenyi), CD13 (R3–242; BD), CD31 (390; BioLegend), MHC1 (28–8-6; BioLegend), and CD16/CD32 (93). Viability was assessed using a Zombie Aqua Fixable Viability kit (cat. no. 423101, BioLegend) or Ghost Dye Violet 510 (cat. no. 13–0870, Tonbo Biosciences).

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Fernández-Castañeda A., Chappell M.S., Rosen D.A., Seki S.M., Beiter R.M., Johanson D.M., Liskey D., Farber E., Onengut-Gumuscu S., Overall C., Dupree J.L, & Gaultier A. (2019). The active contribution of OPCs to neuroinflammation is mediated by LRP1. Acta neuropathologica, 139(2), 365-382.

Publication 2019

CD11b CD62L Ghost Dye Violet 510

Antibodies Cd11b Cd44 Cd62l Flow cytometric Ghost Pdgfrα Tcrβ Violet

Corresponding Organization : University of Virginia

Other organizations : Virginia Commonwealth University

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Variable analysis

independent variables

EBioscience antibodies: TCRβ (H57–597), CD4 (RM4–5), CD8 (53–6.7), CD19 (eBio1D3), CD45 (30-F11), CD11b (M1/70), CD44 (IM7), CD62L (MEL14), CD11c (N418), Ly6G (1A8), PDGFRα (APA5), O4 (O4; Miltenyi), CD13 (R3–242; BD), CD31 (390; BioLegend), MHC1 (28–8-6; BioLegend), and CD16/CD32 (93)

dependent variables

Flow cytometric analyses

control variables

Viability was assessed using a Zombie Aqua Fixable Viability kit (cat. no. 423101, BioLegend) or Ghost Dye Violet 510 (cat. no. 13–0870, Tonbo Biosciences)

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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