NRG-Flk2−/− mice were generated by backcrossing mice harboring a Flk2 null allele (Flt3tm1Irl, kindly provided by Ihor Lemishka, Mount Sinai School of Medicine, NY) for 10 generations to NOD Rag1−/−IL2Rgnull mice (NOD.Cg-Rag1tm1MomIl2rgtm1Wjl/SzJ, obtained from the Jackson Laboratory, catalog number 007799). At each generation, the presence of each mutant allele was confirmed with allele-specific primers. Resultant NRG-Flk2+/− were intercrossed to produce NRG-Flk2−/− mice. NRG-HLA-A2*0201 were generated by intercrossing NSG-A2*0201 (NOD.Cg-PrkdcscidIL2rgtmlWjl/Sz Tg(HLA-A2.1)1Eng/Sz)7 (link),9 (link) with NRG mice. The absence of the SCID mutation and the presence of the Rag1, IL2Rγ null alleles and the A2 transgene was determined by PCR. To generated NRG-Flk2−/− HLA-A*0201 (NFA2) mice, NRGF and NRG-A2 mice were intercrossed. NRG, NRG-A2, NRGF, and NFA2 mice were maintained at the Laboratory Animal Resource Center at Princeton University.
All animal experiments described in this study were performed in accordance with protocols (number 1930) that were reviewed and approved by the Institutional Animal Care and Use and Committee of Princeton University.
All animal experiments described in this study were performed in accordance with protocols (number 1930) that were reviewed and approved by the Institutional Animal Care and Use and Committee of Princeton University.
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