The prostate and prostate cancer cell lines that were used in this experiment, including TrampC1, RM1, CAF and NF, were all derived from Dr. Chang, George Whipple Lab for Cancer Research, and these four types of cells are of mouse origin [16 (link), 17 (link)]. TrampC1 and RM1 were cultured in RPMI-1640 medium (Gibco, Waltham, MA USA) containing 10% foetal bovine serum (Gibco, Waltham, MA USA) and culture conditions of 37 °C with 5% CO2. CAF and NF were cultured in DMEM (Gibco, Waltham, MA USA) containing 10% foetal bovine serum (Gibco, Waltham, MA USA) and incubated at 37 °C with 5% CO2.
In this experiment, human prostate cancer hCAF and human prostate hNF were taken from the primary culture of urological surgical specimens from the Second Hospital of Tianjin Medical University. The hCAF and hNF samples were cultured in DMEM (Gibco, Waltham, MA USA) containing 10% foetal bovine serum (Gibco, Waltham, MA USA) and incubated at 37 °C with 5% CO2.
In this experiment, human prostate cancer hCAF and human prostate hNF were taken from the primary culture of urological surgical specimens from the Second Hospital of Tianjin Medical University. The hCAF and hNF samples were cultured in DMEM (Gibco, Waltham, MA USA) containing 10% foetal bovine serum (Gibco, Waltham, MA USA) and incubated at 37 °C with 5% CO2.
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