Genetically Engineered Mice for Neuronal Labeling

We obtained Gad2-IRES-Cre (Taniguchi et al., 2011 (link), Jax stock #010802), Scnn1a-tg3-cre (Madisen et al., 2010 (link), Jax stock #009613), and Ai14 mice (Madisen et al., 2010 (link), Jax stock #007914) from the Jackson Laboratory. These mice were crossed to generate Gad2-Ai14 and Scnn1a-Ai14 mice. Both sexes were used. In Gad2-Ai14 mice, most cortical inhibitory neurons express tdTomato (Taniguchi et al., 2011 (link)). In Scnn1a-Ai14 mice, layer 4 neurons express tdTomato (Madisen et al., 2010 (link)).

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Hayashi A., Yoshida T, & Ohki K. (2018). Cell Type Specific Representation of Vibro-tactile Stimuli in the Mouse Primary Somatosensory Cortex. Frontiers in Neural Circuits, 12, 109.

Publication 2018

Gad2-IRES-Cre Jax stock Scnn1a-tg3-cre

Cortical Inhibitory Ires Mice Neurons Sexes Tdtomato

Corresponding Organization : Japan Agency for Medical Research and Development

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Variable analysis

independent variables

Crossed Gad2-IRES-Cre mice with Ai14 mice
Crossed Scnn1a-tg3-cre mice with Ai14 mice

dependent variables

Expression of tdTomato in cortical inhibitory neurons in Gad2-Ai14 mice
Expression of tdTomato in layer 4 neurons in Scnn1a-Ai14 mice

control variables

Both sexes were used

controls

Positive control: Gad2-Ai14 mice, where most cortical inhibitory neurons express tdTomato
Positive control: Scnn1a-Ai14 mice, where layer 4 neurons express tdTomato

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