Visualizing Cell Apoptosis with Hoechst

To observe cell apoptosis visually, cells (5 × 10⁴ per well) underwent different treatments were seeded into 6-well plates and stained with Hoechst 33258 solution (Beyotime Company, Shanghai, China) for 5 min. Hoechst 33258 penetrates the plasma membrane and stains DNA blue fluorescent, thereby being used to observe the morphological changes of apoptosis [28 (link), 29 (link)]. The fluorescence microscope was used to observe the morphological changes of cell nuclei.

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Xia F., Chen Y., Jiang B., Bai N, & Li X. (2020). Hsa_circ_0011385 accelerates the progression of thyroid cancer by targeting miR-361-3p. Cancer Cell International, 20, 49.

Publication 2020

Hoechst 33258 solution

Apoptosis Cells Fluorescence microscope Hoechst 33258 Nuclei of cell Plasma membrane Stains

Corresponding Organization :

Other organizations : Xiangya Hospital Central South University, Central South University

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Variable analysis

independent variables

Different treatments applied to the cells

dependent variables

Morphological changes of cell nuclei

control variables

Cell density (5 × 10^4 per well)
Staining with Hoechst 33258 solution for 5 minutes

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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