Immunofluorescence staining was carried out as described previously (Sun et al., 2014 (link)). Frozen tissue sections (6 μm thickness) were incubated in blocking buffer for 2 h at room temperature. After they were washed three times with PBS, sections were incubated with primary antibodies and corresponding secondary antibodies. After washing with PBS for three times, the slides were stained with 4-diamidino-2-phenylindole (DAPI) for 2 min. Images were obtained using a ZEISS HB050 inverted microscope system and the fluorescently stained cells were analyzed using Image J program.
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