Liver samples were collected and immediately fixed in 4% paraformaldehyde. Liver sections were stained with H&E and Masson’s trichrome for analysis of liver granulomas and liver fibrosis, respectively. CD45 (Servicebio, GB11066), CD11b (Servicebio, GB11058) and CD19 (Servicebio, GB11061-1) staining were performed for immunohistochemical analysis, according to standard procedures as our previously study described[9 (link)]. Images were scanned by Pannoramic MIDI II (3D HISTECH) and analyzed with HALO image analysis software (Indica Labs) and CaseViewer software (3D HISTECH).
Lu L., Li T., Feng X., Liu Z., Liu Y., Chao T., Gu Y., Huang R., Zhang F., He L., Zhou B., Kong E., Liu Z., Wang X., Chen Z., Wang H., Malissen M., Malissen B., Zhang L, & Liang Y. (2022). Excessive immunosuppression by regulatory T cells antagonizes T cell response to schistosome infection in PD-1-deficient mice. PLoS Pathogens, 18(6), e1010596.
Standard procedures for immunohistochemical analysis as described in the previous study[9]
controls
No positive or negative controls were explicitly mentioned.
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