Evaluating Bone Formation in Cranium

Excised samples in the cranium were scanned with micro-CT (Siemens Inveon, Germany) to evaluate new bone formation within the implant sites. The scan was at energy of 80 kV and 500 μA, a current of 145 mA, and an isotropic voxel size of 27 μm. After 3D reconstruction, quantitative analyses of bone volume/total volume (BV/TV), trabecular thickness (Tb.Th), trabecular separation/spacing (Tb.Sp) and trabecular number (Tb.N) in the same region of interest within defects were performed using Inveon Research Workplace 2.2 [46 (link)].

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Zang S., Zhu L., Luo K., Mu R., Chen F., Wei X., Yan X., Han B., Shi X., Wang Q, & Jin L. (2017). Chitosan composite scaffold combined with bone marrow-derived mesenchymal stem cells for bone regeneration: in vitro and in vivo evaluation. Oncotarget, 8(67), 110890-110903.

Publication 2017

micro-CT

A 145 Bone Bone formation Cranium Reconstruction Scan Scanned ct Trabecular

Corresponding Organization : Southern Medical University

Other organizations : Air Force Medical University, Urumqi General Hospital of PLA

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Variable analysis

independent variables

Implant sites

dependent variables

Bone volume/total volume (BV/TV)
Trabecular thickness (Tb.Th)
Trabecular separation/spacing (Tb.Sp)
Trabecular number (Tb.N)

control variables

Scan energy of 80 kV and 500 μA
Scan current of 145 mA
Isotropic voxel size of 27 μm

controls

No positive or negative controls were explicitly mentioned.

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