Characterization of Cancer Cell Lines

The human lung cancer A549, colon cancer SW620, osteosarcoma cancer HOS and 143B cell lines were purchased from the American Type Culture Collection (ATCC). The human prostate cancer PC-3U cells represents a clone from the original PC-3 cell line (ATCC CRL-1435)^{54 (link)}. The human glioblastoma U251 MG (formerly U-373 MG) originated from the European Collection of Authenticated Cell Cultures (ECACC). The four cancer cell lines stably expressing GFP; U251-GFP, SW620-GFP, PC-3U-GFP, and A549-GFP, were cultured in RPMI media supplemented with 10% (v/v) FBS, and 1% (v/v) PS (Gibco). Cells were cultured at 37 °C in the presence of 5% (v/v) CO2. Cancer cells were maintained under passage 15. Mycoplasma test (Eurofins GATC Biotech) confirmed that the cancer cells were free from mycoplasma infection, and the identity of the cancer cell lines was verified by STR profiling (Microsynth).