Lung-only Mice (LoM) Model for HCMV

Lung-only mice (LoM) were generated as previously described (67 (link), 68 (link)). In brief, LoM were constructed by implanting of 2 pieces of human lung tissue (Advanced Bioscience Resources) subcutaneously into the back of male and female NOD.Cg-Prkdc^scidIl2rg^tm1Wjl/SzJ mice (NSG, The Jackson Laboratory). Expansion of the implants was monitored by palpation. Anesthetized mice were exposed to HCMV by direct injection of HCMV TB40/E (4.25 × 10⁵ IU) into the implants in a total volume of 100 μL. Mice received vehicle control (0.5% methylcellulose, 0.5% Tween-80; p.o., b.i.d.), FLS-359 (50 mg/kg in vehicle; p.o., b.i.d.), or ganciclovir (100 mg/kg; i.p., daily) beginning 2 hours before infection. Human lung implants were harvested at 17 dpi and flash-frozen. Subsequently, implants were thawed and homogenized, and virus load was measured by TCID₅₀ assay.

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Roche K.L., Remiszewski S., Todd M.J., Kulp JL I.I.I., Tang L., Welsh A.V., Barry A.P., De C., Reiley W.W., Wahl A., Garcia J.V., Luftig M.A., Shenk T., Tonra J.R., Murphy E.A, & Chiang L.W. (2023). An allosteric inhibitor of sirtuin 2 deacetylase activity exhibits broad-spectrum antiviral activity. The Journal of Clinical Investigation, 133(12), e158978.

Publication 2023

NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ mice

Assay Female Frozen Ganciclovir Hcmv Human Infection Lung Male Methylcellulose Mice Nod mice Palpation Tissue Tween 80

Corresponding Organization :

Other organizations : Duke University, Trudeau Institute, Princeton University, SUNY Upstate Medical University

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Variable analysis

independent variables

Administration of vehicle control (0.5% methylcellulose, 0.5% Tween-80; p.o., b.i.d.)
Administration of FLS-359 (50 mg/kg in vehicle; p.o., b.i.d.)
Administration of ganciclovir (100 mg/kg; i.p., daily)

dependent variables

Virus load in human lung implants measured by TCID50 assay

control variables

Lung-only mice (LoM) generated by implanting 2 pieces of human lung tissue subcutaneously into the back of male and female NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice
Exposure of mice to HCMV by direct injection of HCMV TB40/E (4.25 × 105 IU) into the implants
Timing of treatment administration (beginning 2 hours before infection)

positive control

Not explicitly mentioned

negative control

Administration of vehicle control (0.5% methylcellulose, 0.5% Tween-80; p.o., b.i.d.)

Annotations

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