In Vitro Expansion of Glioma Stem Cells

For in vitro expansion, GSCs were cultured in Neurobasal A media (NBA; 10888-022, Gibco, Waltham, MA, USA) supplemented with N2 and B27 (0.5× each; 17502-048 and 12587-010, Gibco), human recombinant basic fibroblast growth factor, and epidermal growth factor (20 ng/mL each; 233-FB-001MG/CF and 236-EG-01M, R&D Systems, Minneapolis, MN, USA), as well as 100× penicillin streptomycin-glutamine (10378-016, Gibco) [7 (link)]. Next, GSCs were cultured at 37 °C in a humidified incubator containing 5% CO₂.

Free full text: Click here

Lee M., Kim Y.S., Lee K., Kang M., Shin H., Oh J.W., Koo H., Kim D., Kim Y., Kong D.S., Nam D.H, & Lee H.W. (2019). Novel Semi-Replicative Retroviral Vector Mediated Double Suicide Gene Transfer Enhances Antitumor Effects in Patient-Derived Glioblastoma Models. Cancers, 11(8), 1090.

Publication 2019

Neurobasal A media 233-FB-001MG/CF 236-EG-01M

Basic fibroblast growth factor Epidermal growth factor Glutamine Human Penicillin Streptomycin

Corresponding Organization : Samsung (South Korea)

Other organizations : Chungnam National University

Top 5 similar protocols

Variable analysis

independent variables

Culture media (Neurobasal A media)
Supplements (N2, B27, human recombinant basic fibroblast growth factor, epidermal growth factor)
Antibiotics (penicillin streptomycin-glutamine)

dependent variables

In vitro expansion of GSCs

control variables

Temperature (37 °C)
Atmosphere (humidified incubator containing 5% CO2)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!