Heat Stress Tolerance Evaluation in Crops

Analysis of variance (ANOVA) for alpha lattice design of all studied traits in each location was performed in GenStat 18th edition.¹ We used Tukey’s honestly significant difference (HSD) test for environment–environment comparisons. Pearson’s correlation coefficient between traits in each environment was calculated in IBM SPSS Statistics for Windows v. 25 (IBM Corp., Armonk, NY, United States). Broad-sense heritability was estimated in Plant Breeding Tools v. 1.4.²To identify heat stress-tolerant genotypes, heat tolerance efficiency (HTE) was calculated as 100*(Y_si/Y_pi), where Y_si is GY under stress or in a hot environment and Y_pi is GY under an optimum or cold environment (Elbashir et al., 2017 (link)). In the first HTE (HTE1), we used the GY values from DON as the cold environment and MED/SD1 as the hot environment. In the second HTE (HTE2), we considered GY values from MED/SD1 as the cold environment and MED/SD2 as the hot environment.