Identification of Fungal Metabolites by UHPLC-HRMS

The secondary metabolites isolated in IC and EC extracts of F. oxysporum were identified by UHPLC-HRMS. Analyses were performed on an Agilent 1290 Infinity LC system with a DAD detector, coupled to an Agilent 6550 iFunnel Q-TOF with an electrospray ionization source (Agilent Technologies, Santa Clara, CA, USA). The separation was performed on a 2.1 mm i.d. × 250 mm, 2.7 μm Poroshell 120 Phenyl-Hexyl column (Agilent) at 60 °C with a water-acetonitrile gradient (both buffered with 20 mM formic acid), according to the method described by Klitgaard et al. (2014 (link)).

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Lebeau J., Petit T., Dufossé L, & Caro Y. (2019). Putative metabolic pathway for the bioproduction of bikaverin and intermediates thereof in the wild Fusarium oxysporum LCP531 strain. AMB Express, 9, 186.

Publication 2019

1290 Infinity LC system Agilent 6550 iFunnel Q-TOF Poroshell 120 Phenyl-Hexyl column

Acetonitrile Formic acid

Corresponding Organization :

Other organizations : Observatoire des Sciences de l'Univers de la Réunion

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Variable analysis

independent variables

Extraction method (IC and EC)

dependent variables

Identification of secondary metabolites in the extracts

control variables

UHPLC-HRMS analytical method (Agilent 1290 Infinity LC system with a DAD detector, coupled to an Agilent 6550 iFunnel Q-TOF with an electrospray ionization source)
Chromatographic separation conditions (2.1 mm i.d. × 250 mm, 2.7 μm Poroshell 120 Phenyl-Hexyl column at 60 °C with a water-acetonitrile gradient buffered with 20 mM formic acid)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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