Assessing Cell Migration and Invasion

Migration and invasion of MM cells was assessed as described before [24 (link)]. Briefly, MSTO-wt and MSTO-shCALB2-IPTG (induced) cells were grown to confluence in 96-well ImageLock plates (Essen Bioscience) pre-coated with a thin layer of 0.1 mg/mL Matrigel Basement Membrane Matrix (Corning, Oneonta, NY, USA, Cat. No.354234). A scratch of about 1 mm was created using the 96 well-plate woundmaker tool (Essen Bioscience) as described by the manufacturer. In the case of the invasion assay, 50 µL of Matrigel (0.6 mg/mL) was added to each well after the scratch. After 30 min incubation, 100 µL of medium was added on top and plates were scanned at a 2 h-frequency using the Incucyte™ system (Essen Bioscience). Images were evaluated with the IncuCyte™ software system (version 2011A, Essen Bioscience).