Preparation and Storage of Pharmacological Compounds

The following drugs were dissolved in DMSO and stored at −20°C: WEHI-539 [32 (link)] (Apexbio); taxol (Sigma); nocodazole (Sigma); AZ138 [8 (link)] (AstraZeneca)); AZ3146 [43 (link)] (AstraZeneca); BI 2536 [38 (link)] (Boehringer Ingelheim); GSK923295 [39 (link),70 ]; MLN8054 [41 (link)] (Millennium Pharmaceuticals); ZM447439 [42 (link)] (Tocris); A-1210477 [31 (link)] (Medchemexpress). Tetracycline (Sigma) was dissolved in water, stored at −20°C, and used at concentrations indicated in the figure legends. Thymidine (Sigma) was dissolved in PBS at a concentration of 200 mM, and stored short-term at 4°C.

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Bennett A., Sloss O., Topham C., Nelson L., Tighe A, & Taylor S.S. (2016). Inhibition of Bcl-xL sensitizes cells to mitotic blockers, but not mitotic drivers. Open Biology, 6(8), 160134.

Publication 2016

WEHI-539 taxol nocodazole AZ138 BI 2536 ZM447439 A-1210477 Tetracycline Thymidine

Bi 2536 Dmso Gsk923295 Mln8054 Nocodazole Pharmaceuticals Taxol Tetracycline Thymidine Zm447439

Corresponding Organization :

Other organizations : Google (United States)

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Variable analysis

independent variables

WEHI-539
Taxol
Nocodazole
AZ138
AZ3146
BI 2536
GSK923295
MLN8054
ZM447439
A-1210477
Tetracycline
Thymidine

dependent variables

Not explicitly mentioned

control variables

Solvent (DMSO) used to dissolve drugs
Storage temperature (-20°C) for drug solutions
Water used to dissolve tetracycline
PBS used to dissolve thymidine

positive controls

None specified

negative controls

None specified

Annotations

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