The antibodies used were as follows: anti-c-Myc antibody (9E10, 1:1000 for IB analysis, Santa Cruz), anti-Flag antibody (F1804, 1:1000 for IB analysis, Sigma), anti-HA antibody (1:5000 for IB analysis, Covance), anti-GAPDH antibody (SC-47724, 1:1000 for IB analysis, Santa Cruz), anti-α-tubulin antibody (EPR1333, 1:10 000 for IB analysis, Epitomics), anti-HIF-2α antibody (AB199, 1:1000 for IB analysis, Abcam), anti-HIF-1α antibody (A6265, 1:1000 for IB analysis, Abclone), anti-HIF-OH antibody (3434S, 1:1000 for IB analysis, Cell Signaling).
Western blot was performed as described previously (31 (link)). The Fuji Film LAS4000 mini luminescent image analyzer was used for photographing the blots. Multi Gauge V3.0 was used for quantifying the protein levels based on the band density obtained in western blot analysis.
Western blot was performed as described previously (31 (link)). The Fuji Film LAS4000 mini luminescent image analyzer was used for photographing the blots. Multi Gauge V3.0 was used for quantifying the protein levels based on the band density obtained in western blot analysis.
