Immunohistochemistry was performed using the avidin-biotin-peroxidase complex method (Ultrasensitive; MaiXin, Fuzhou, China). CD31 mouse monoclonal antibody (1:300 dilution; Abcam, Inc., Cambridge, UK), anti-human leukocyte antigen mAb (1:100 dilution; Abcam Inc.), and mouse immunoglobulin (a negative control) were used as previously described [16 (link)]. Hematoxylin was used for counterstaining.
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