Total proteins were isolated from parent and Cat D KO Caki cancer cells according to a previously described method17 (link),18 (link). Briefly, cells were loaded onto silica capillary columns containing 7.5 cm of 5 lm particle Aqua C18 reversed-phase column material. The column was placed in-line with an Agilent HP1100 quaternary LC pump, and a splitter system was used to achieve a flow rate of 250 nL/min. Peptides were eluted and electrosprayed into an LTQ Ion Trap mass spectrometer (Thermo Finnigan, Palo Alto, CA, USA). MS/MS spectra were individually matched with the human IPI protein using the TurboSEQUEST and SEQUEST Cluster Systems.
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