RT-qPCR was performed on cDNA isolated from adult hypothalamus using the Quantinova SYBR green kit (QIAGEN #208056) on a Quantstudio 6 thermocycler (Applied Biosystems). Data was analysed using the relative standard curve method as previously described (Scagliotti et al., 2021 (link)). Actb expression was used to normalise the expression of the target genes. Details of the primers used in this study are included in Supplementary file 1i.
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