Evaluating Ternary Solubility Enhancement

A phase solubility study was conducted to evaluate the Ks and Ce of ternary mixtures PTS/βCD/PLF. In the present study, excessive quantities of PTS were added to aqueous solution having different concentrations of βCD from 2 mM to 8 mM and PLF (10%). The samples were placed in water shaker bath for seventy-two hours at 25 °C [60 (link),61 (link),62 (link),63 (link)]. After 72 h, the supernatant from each sample was carefully pipetted out and filtered through 0.45 μm membrane filters (“Chromafil^®Xtra, Macherey-Nagel GmbH & Co. KG, Düren, Germany”). The drug content in the samples was analyzed by UV spectrophotometer at 318 nm [64 (link),65 (link)]. According to Equation (1), a slope-based calculation of Ks can be performed using the phase solubility plot [66 (link),67 (link),68 ,69 (link)]. Equation (2) was used to calculate Ce for each sample [66 (link),70 (link),71 (link)]. S₀ is the equilibrium solubility of PTS in water [72 (link),73 (link)].
$$Ks=\frac{Slope}{Intercept (1-Slope)}$$
$$C_e=S_{0}Ks$$