Measuring Brain Evans Blue Extravasation

At the 14 days, administered (Cohort 2) i.p. with 3% Evans Blue (EB; Sigma-Aldrich, USA) as described previously (39 (link)). Four hours after post-injection, the minks were sacrificed. The brains were harvested and fixed in 4% formalin (BBI Life Sciences Corporation, China). Frozen minks brain slabs (6 μm) are kept at cryogenic temperatures, and sections were examined in OLYMPUS Digital Pathology (OLYMPUS, Japan). ImageJ software was used as the integrated density analysis tool to measure extravascular accumulations of EB. Three micrographs per individual were performed for the photomicrographs.

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Liu C., Guo X., Si H, & Li G. (2022). A mink (Neovison vison) model of self-injury: Effects of CBP-CREB axis on neuronal damage and behavior. Frontiers in Veterinary Science, 9, 975112.

Publication 2022

Digital Pathology

Brain Evans blue Formalin Frozen Minks Photomicrographs

Corresponding Organization : Qingdao Agricultural University

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Variable analysis

independent variables

Administration of 3% Evans Blue (EB) intraperitoneally (i.p.) to Cohort 2 at 14 days

dependent variables

Extravascular accumulations of EB measured using ImageJ software

control variables

Minks sacrificed 4 hours after EB injection
Brains harvested and fixed in 4% formalin
Frozen brain slabs (6 μm) kept at cryogenic temperatures
Sections examined using OLYMPUS Digital Pathology

controls

No positive or negative controls were explicitly mentioned in the provided information.

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