Preparation of LCWB Samples for SEM

The treated and untreated LCWB samples were fixed with glutaraldehyde, dehydrated with ascending concentrations of ethanol, then immersed in hexamethyldisilazane (HMDS, Sigma-Aldrich, Milan, Italy) for 10 min, twice. HMDS was decanted from the specimen vial and the tissues were left to air dry at room temperature. The dried samples were subjected to gold-sputtering with a desk sputter coater (Phenom-World B.V., The Netherlands) and then observed with SEM (Phenom-World B.V., The Netherlands) at different magnifications, as previously described [23 (link),24 (link)].

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Di Fermo P., Ciociola T., Di Lodovico S., D’Ercole S., Petrini M., Giovati L., Conti S., Di Giulio M, & Cellini L. (2021). Antimicrobial Peptide L18R Displays a Modulating Action against Inter-Kingdom Biofilms in the Lubbock Chronic Wound Biofilm Model. Microorganisms, 9(8), 1779.

Publication 2021

desk sputter coater

Dehydrated ethanol Glutaraldehyde Gold Hexamethyldisilazane Hmds Tissues

Corresponding Organization :

Other organizations : University of Chieti-Pescara, University of Parma

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Variable analysis

independent variables

Treatment (treated vs. untreated LCWB samples)

dependent variables

Morphological characteristics of the LCWB samples observed using SEM

control variables

Glutaraldehyde fixation
Dehydration with ascending concentrations of ethanol
Immersion in hexamethyldisilazane (HMDS)
Air drying at room temperature
Gold-sputtering with a desk sputter coater

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