Primary RCFs were isolated as previously described and used between passages 3 and 7 [26 (link),27 (link)]. RCFs were cultured in Dulbecco’s modified medium (DMEM) low glucose with 10% fetal bovine serum (Atlanta Biologicals, Lawrence, GA, USA) and 1% penicillin-streptomycin-amphotericin B (PSF; Lonza, Walkersville, MD, USA).
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