Tissue NO Content and Metabolic Assessment

Tissue NO content was measured by using an electron spin resonance spectroscopy (ESR) technique, as previously described (21 (link),31 (link)). Insulin levels were determined using a mouse insulin ELISA kit (Crystal Chem, Downers Grove, IL). Determinations of body lean and fat mass were made in conscious mice using quantitative magnetic resonance (EchoMRI 3-in-1 body composition analyzer; Echo Medical Systems, Houston, TX) in the University of Washington Nutrition Obesity Research Center. Hepatic triglyceride (TG) content was enzymatically measured in liver lysates as previously described (32 (link)).

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Lee W.J., Tateya S., Cheng A.M., Rizzo-DeLeon N., Wang N.F., Handa P., Wilson C.L., Clowes A.W., Sweet I.R., Bomsztyk K., Schwartz M.W, & Kim F. (2015). M2 Macrophage Polarization Mediates Anti-inflammatory Effects of Endothelial Nitric Oxide Signaling. Diabetes, 64(8), 2836-2846.

Publication 2015

mouse insulin ELISA kit EchoMRI 3-in-1 body composition analyzer

Body Body composition Conscious Echo Electron spin resonance spectroscopy Elisa Insulin Liver Magnetic resonance Mice Obesity Tissue Triglyceride

Corresponding Organization : University of Washington

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Variable analysis

independent variables

Tissue NO content

dependent variables

Insulin levels
Body lean and fat mass
Hepatic triglyceride (TG) content

control variables

Conscious mice

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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