RNA Extraction and qPCR Normalization

RNA was extracted using an RNA extraction kit (Qiagen, Chatsworth, CA). qPCR reagents were from Applied Biosystems (Foster City, CA). Ribosomal protein 36B4 was used to normalize gene expression (Li et al., 2013 (link)).

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Li Y., Lei D., Swindell W.R., Xia W., Weng S., Fu J., Worthen C.A., Okubo T., Johnston A., Gudjonsson J.E., Voorhees J.J, & Fisher G.J. (2015). Age-associated increase of skin fibroblast-derived prostaglandin E2 contributes to reduced collagen levels in elderly human skin. The Journal of investigative dermatology, 135(9), 2181-2188.

Publication 2015

RNA extraction kit qPCR reagents

Gene expression Ribosomal protein

Corresponding Organization :

Other organizations : University of Michigan–Ann Arbor

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Gene expression

control variables

Ribosomal protein 36B4 (used to normalize gene expression)

controls

Positive control: not mentioned
Negative control: not mentioned

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