MESA Receptor Cloning in HEK293FT

MESA receptors were cloned into pcDNA backbones to confer high expression in HEK293FT cells. These plasmid backbones are versions of the pcDNA3.1/Hygro(+) Mammalian Expression Vector (Thermo Fisher #V87020), modified by our laboratory in previous work (Addgene #138749) (32 (link)). In general, restriction sites were chosen to facilitate modular swapping of parts via restriction enzyme cloning. A complete list of plasmids used in this study is provided in Supplementary Data S1. Plasmid maps are included as GenBank files in Supplementary Data S2.

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Edelstein H.I., Donahue P.S., Muldoon J.J., Kang A.K., Dolberg T.B., Battaglia L.M., Allchin E.R., Hong M, & Leonard J.N. (2020). Elucidation and refinement of synthetic receptor mechanisms. Synthetic Biology, 5(1), ysaa017.

Publication 2020

Mammalian Expression Vector

Backbones Cells Mammalian Maps Plasmid Restriction enzyme Vector

Corresponding Organization : Northwestern University

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Variable analysis

independent variables

MESA receptors

dependent variables

Expression of MESA receptors in HEK293FT cells

control variables

PcDNA backbones (versions of the pcDNA3.1/Hygro(+) Mammalian Expression Vector)

controls

Positive control: Not specified
Negative control: Not specified

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