2-(N-morpholino)ethanesulfonic acid (MES) was purchased from EMD-Calbiochem (Gibbstown, NJ). Adenosine, adenosine 5′-diphosphoribose (ADP ribose), adenosine 5′-monophosphate (AMP), p-aminohippuric acid, 1-ethyl-3-(3-methylaminopropyl)carbodiimide (EDC), gluteraldehyde, hydroxylamine hydrochloride, nicotinamide, nicotinamide adenine dinucleotide (NAD+), nicotinamide mononucleotide (NMN), potassium phosphate dibasic, pyridine (99.8%), sodium azide, sodium cyanoborohydride, and sodium phosphate monobasic were obtained from Sigma-Aldrich Chemical Co. (Milwaukee, WI). N-Hydroxysulfosuccinimide (Sulfo-NHS) was from Pierce (Rockford, IL). Acetylated histone H3 (K9) peptide (H3K9Ac) and acetylated histone H3 (K14) peptide (H3K14Ac) were from Upstate/Millipore (Temecula, CA). Solutions were prepared using purified water from a Millipore MilliQ system (Millipore Corporation, Bedford, MA). BcMag amine-terminated magnetic beads (50 mg/mL, 1 µm) were purchased from Bioclone, Inc. (San Diego, CA). The manual magnetic separator Dynal MPC-S was from Invitrogen Corporation (Carlsbad, CA).
Protocol detail
Histone Acetylation Detection Protocol
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2 n morpholino ethanesulfonic acid
Adenosine
Adenosine 5 monophosphate
Adp ribose
Amine
Carbodiimide
Histone h3
Hydroxylamine hydrochloride
N hydroxysulfosuccinimide
Nicotinamide
Nicotinamide adenine dinucleotide
Nicotinamide mononucleotide
P aminohippuric acid
Peptide
Potassium phosphate dibasic
Pyridine
Sodium azide
Sodium cyanoborohydride
Sodium phosphate
Sulfo nhs
Corresponding Organization :
Other organizations : Institute on Aging, National Institute on Aging, National Institutes of Health
Top 5 similar protocols
Protocol cited in 5 other protocols
Variable analysis
independent variables
- 1-ethyl-3-(3-methylaminopropyl)carbodiimide (EDC)
- Gluteraldehyde
- Hydroxylamine hydrochloride
- Sodium cyanoborohydride
- Acetylated histone H3 (K9) peptide (H3K9Ac)
- Acetylated histone H3 (K14) peptide (H3K14Ac)
- BcMag amine-terminated magnetic beads
- Not explicitly mentioned
- 2-(N-morpholino)ethanesulfonic acid (MES)
- Adenosine
- Adenosine 5′-diphosphoribose (ADP ribose)
- Adenosine 5′-monophosphate (AMP)
- P-aminohippuric acid
- Nicotinamide
- Nicotinamide adenine dinucleotide (NAD+)
- Nicotinamide mononucleotide (NMN)
- Potassium phosphate dibasic
- Pyridine (99.8%)
- Sodium azide
- Sodium phosphate monobasic
- N-Hydroxysulfosuccinimide (Sulfo-NHS)
- Purified water from a Millipore MilliQ system
- Manual magnetic separator Dynal MPC-S
- Not explicitly mentioned
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