Protocol detail

Dual-channel Imaging of Cytoskeletal Dynamics

Find Similar Protocols

UAS-Lifeact-Ruby (Bloomington stock 35545), BAC-encoded aPKC-GFP (Besson et al., 2015 (link)) and Sqh-GFP (Royou et al., 2002 (link)) transgenes were used to assess F-actin, aPKC and myosin II dynamics, respectively. Expression of Lifeact was specifically driven in nerve cells upon crossing UAS-Lifeact-Ruby to insc-Gal4 (1407-Gal4, Bloomington stock 8751) or to worniu-Gal4 (Bloomington stock 56553). The following genotypes were examined through dual channel live imaging: BAC-aPKC-GFP / Y; insc-Gal4, +/+, UAS-Lifeact-Ruby; and; worGal4, Sqh-GFP, +/+, UAS-Lifeact-Ruby.

Free full text: Click here

Oon C.H, & Prehoda K.E. (2021). Phases of cortical actomyosin dynamics coupled to the neuroblast polarity cycle. eLife, 10, e66574.

Publication 2021

UAS-Lifeact-Ruby 1407-Gal4 worniu-Gal4

F actin Genotypes Myosin ii Nerve cells Transgenes

Top 5 similar protocols

Variable analysis

independent variables

Crossing UAS-Lifeact-Ruby to insc-Gal4 (1407-Gal4, Bloomington stock 8751) or to worniu-Gal4 (Bloomington stock 56553) to drive expression of Lifeact in nerve cells

dependent variables

F-actin dynamics
APKC dynamics
Myosin II dynamics

control variables

BAC-encoded aPKC-GFP
Sqh-GFP

controls

Positive control: BAC-aPKC-GFP / Y; insc-Gal4, +/+, UAS-Lifeact-Ruby
Positive control: worGal4, Sqh-GFP, +/+, UAS-Lifeact-Ruby

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!