Sequencing of Viral Genomes from Insect Hosts

H. magnanima female adults of the male-killing strain (hereinafter referred to as the late strain) were homogenized in PBS as above and passed through a 0.45 μm filter. The filtrates were treated with RNase A and DNase I as described previously (Fujita et al., 2016 (link)); then RNA was extracted using Isogen II (Nippon Gene). Construction of a cDNA library and sequencing analysis was carried out according to the method described in Fujita et al. (2016 (link)). Reads were analyzed with CLC Genomic Workbench software (CLC bio) or the SPAdes algorithm (Bankevich et al., 2012 (link)). Complete OGV genomic sequences were determined by the FLDS sequencing method as described previously (Urayama et al., 2016 (link)). Complete OGV nucleotide sequences were submitted to the DDBJ/GenBank/EMBL database under accession numbers LC383810-LC383814 and LC597875-LC597896.