Cell cultures on 6-well MEAs (Multi-Channel Systems) were recorded in two methods detailed in Supplementary Fig. S12 . Recordings typically totaled 4–6 min. A temperature-controlled plate was used to keep cell cultures at 37C. Electrodes were allowed to equilibrate for approximately 5 min before starting recordings. MC_Rack software from Multi Channel Systems collected digital voltage trace recordings from the MEAs.
Two methods were developed to elicit and identify changes in electrophysiological functioning to compare stimulated activity. The first method utilized 100 µM dopamine hydrochloride (cat: H8502-25G, Sigma) and 1 µM acetylcholine chloride (cat: A2661-25G, Sigma) with an hour washout incubation between stimuli to determine whether PFF dosage impacts response differs depending on the neurotransmitter66 (link),67 (link). The second method used a square excitation waveform frequency of 2000 ms and 1200 mV excitation for 1000 ms for electrical stimulation based on previous literature, with a dopamine dosage applied immediately following54 (link),55 (link). MEA recordings were preprocessed in MC_Rack (Multi-Channel Systems) using a bandpass filter of 200–3000 Hz to reduce noise, artifacts, and local field potentials. The spike detection function was used to identify spiking events at a threshold of − 5 standard deviations.
Two methods were developed to elicit and identify changes in electrophysiological functioning to compare stimulated activity. The first method utilized 100 µM dopamine hydrochloride (cat: H8502-25G, Sigma) and 1 µM acetylcholine chloride (cat: A2661-25G, Sigma) with an hour washout incubation between stimuli to determine whether PFF dosage impacts response differs depending on the neurotransmitter66 (link),67 (link). The second method used a square excitation waveform frequency of 2000 ms and 1200 mV excitation for 1000 ms for electrical stimulation based on previous literature, with a dopamine dosage applied immediately following54 (link),55 (link). MEA recordings were preprocessed in MC_Rack (Multi-Channel Systems) using a bandpass filter of 200–3000 Hz to reduce noise, artifacts, and local field potentials. The spike detection function was used to identify spiking events at a threshold of − 5 standard deviations.
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