RNA Resistance Assay with RNase R
Corresponding Organization : Kyoto University
Variable analysis
- Incubation time (45 min)
- Circular EGFP ΔpAΔIRES RNA structure (detected by denaturing PAGE and SYBR Green II staining)
- Amount of in vitro-transcribed Circular EGFP ΔpAΔIRES RNA (2.5 μg)
- Concentration of RNase R enzyme (10 U)
- Incubation temperature (37°C)
- Denaturing PAGE conditions (8.3 M urea, 4% and 8% gels)
Annotations
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