The cells were digested into a single-cell suspension. Annexin V-phycoerythrin/7-amino-actinomycin D (PE/7AAD; KeyGEN BioTECH, Nanjing, China) was added according to the manufacturer’s instructions. The cells were incubated at room temperature for 15 min in the dark. They were then analyzed by flow cytometry (BD FACSCalibur; BD Biosciences, NJ, USA).
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