Animal Model for Behavioral Studies

Male C57Bl/6N mice purchased from SLC (Shizuoka, Japan) were maintained in a temperature- and humidity-controlled room with a 12-h light/dark cycle and free access to food and water. Animals associated with tissue or cell sampling were euthanized by cervical dislocation after intraperitoneal injection of an anesthetic mixture consisting of medetomidine (0.3 mg/kg, Meiji Seika Pharma, Tokyo, Japan), midazolam (4 mg/kg; Sandoz, Tokyo, Japan), and butorphanol (5 mg/kg, Meiji Seika Pharma); VPA was purchased from Sigma-Aldrich (St. Louis, MO, USA). All animal care and experimental procedures complied with the regulations for animal experiments and related activities of the Tohoku University. This study was approved by the Tohoku University Institutional Animal Care and Use Committee. This study was conducted in accordance with the ARRIVE guidelines.