Tumor Specimen Processing and Storage

Tumor specimen will be cut into 1-3 mm³ pieces. Depending on the quantity of tumor, different procedures will be carried out in the following order of priority: 1) one random piece will be processed for the isolation of viable cells and PDTO establishment; 2) two random pieces will be snap frozen and stored at − 80 °C for molecular analyses; 3) One random piece will be fixed in paraformaldehyde for paraffin embedding and subsequent histopathological analysis and immunohistochemistry; 4) the remainder will be cryopreserved in freezing solution (10% DMSO, 90% non-inactivated FBS) for future isolation of viable cells. All tumor samples will be stored in the Biological Resource Center ‘Tumorotheque de Caen Basse-Normandie’.