Aconitase Activity Assay in FRDA Tissues

Aconitase activities of YG8JR and Y47JR heart and cerebellum tissues (n = 8 each group) were determined using the Aconitase assay kit (Cayman Chemical Company, MI, United States) and normalized to citrate synthase activities determined using a Citrate Synthase assay kit (Sigma, MO, United States), as previously described (Sandi et al., 2014 (link); Anjomani Virmouni et al., 2015b (link)). All experiments were performed in triplicates and protein concentrations were determined using Pierce BCA assay (Thermo Fisher). The absorbance detection was carried out using CLARIOstar Microplate Reader (BMG LABTECH, Aylesbury, United Kingdom).

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Kalef-Ezra E., Edzeamey F.J., Valle A., Khonsari H., Kleine P., Oggianu C., Al-Mahdawi S., Pook M.A, & Anjomani Virmouni S. (2023). A new FRDA mouse model [Fxnnull:YG8s(GAA) > 800] with more than 800 GAA repeats. Frontiers in Neuroscience, 17, 930422.

Publication 2023

Aconitase assay kit Citrate Synthase assay kit Pierce BCA assay CLARIOstar Microplate Reader

Aconitase Assay Cayman Cerebellum Citrate synthase Heart Protein Tissues

Corresponding Organization : Brunel University of London

Other organizations : Research Institute of Health Sciences, Health Research Institute of the Balearic Islands, Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition, Instituto de Salud Carlos III

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Variable analysis

independent variables

Mouse genotype (YG8JR and Y47JR)

dependent variables

Aconitase activities in heart and cerebellum tissues

control variables

Citrate synthase activities in heart and cerebellum tissues
Protein concentrations determined using Pierce BCA assay
Absorbance detection using CLARIOstar Microplate Reader

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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