Macrophage Differentiation and Ox-LDL Exposure

THP-1 monocyte-like cells (American Type Culture Collection) were cultured in RPMI-1640 medium supplemented with 10% FBS, 2 mM L-glutamine and 1% penicillin/streptomycin (all Gibco) at 37°C with 5% CO₂. THP-1 cells were differentiated into macrophages by exposure to 100 nM PMA (Sigma-Aldrich) as previously described [21 (link)] for indicated hours. Then cells were incubated with 50 μg/ml ox-LDL for 24 h. For NRF2 inhibition, 2 μM ML385 (Sigma-Aldrich) was used to treat cells for 24 h.

Free full text: Click here

Mu F., Wang Y., Wu H., You Q, & Zhang D. (2021). The myocardial infarction-associated transcript 2 inhibits lipid accumulation and promotes cholesterol efflux in oxidized low-density lipoprotein-induced THP-1-derived macrophages via inhibiting mitogen-activated protein kinase signaling and activating the nuclear factor erythroid-related factor 2 signaling pathway. Bioengineered, 13(1), 407-417.

Publication 2021

RPMI-1640 medium FBS L-glutamine penicillin/streptomycin PMA ML385

Cells Cultured medium Glutamine Inhibition Macrophages Monocyte Nrf2 Ox ldl Penicillin Streptomycin Thp 1 cells

Corresponding Organization : Nanjing Medical University

Other organizations : First Affiliated Hospital of Chongqing Medical University, Chongqing Medical University, Second Affiliated Hospital of Chongqing Medical University, Dalian Medical University, Army Medical University, Southwest Hospital

Top 5 similar protocols

Variable analysis

independent variables

Exposure to 100 nM PMA for indicated hours
Incubation with 50 μg/ml ox-LDL for 24 h
Treatment with 2 μM ML385 for 24 h

dependent variables

Differentiation of THP-1 cells into macrophages
Cellular response to ox-LDL exposure
Effect of NRF2 inhibition on cellular response

control variables

Culture of THP-1 monocyte-like cells in RPMI-1640 medium supplemented with 10% FBS, 2 mM L-glutamine and 1% penicillin/streptomycin
Incubation at 37°C with 5% CO2

controls

Positive control: THP-1 cells differentiated into macrophages by exposure to 100 nM PMA
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!