Protocol detail

Protein Extraction and Antibody Analysis

Find Similar Protocols

Protein was extracted from liver tissue or cell cultures as described (27 (link)). The monoclonal rabbit anti-mouse Notch1, NICD, Hes1, p-JNK, JNK, ROCK1, PTEN, p-Akt, Akt, TLR4, cleaved caspase-3, p-IκBα, and β-actin Abs (Cell Signaling Technology, MA) and mouse monoclonal antibody JSAP1 (Santa Cruz Biotechnology) were used. See Supplementary Materials.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Lu L., Yue S., Jiang L., Li C., Zhu Q., Ke M., Lu H., Wang X., Busuttil R.W., Ying Q.L., Kupiec-Weglinski J.W, & Ke B. (2018). Myeloid Notch1 Deficiency Activates RhoA/ROCK Pathway And Aggravates Hepatocellular Damage In Mouse Ischemic Livers. Hepatology (Baltimore, Md.), 67(3), 1041-1055.

Publication 2017

p-JNK ROCK1 p-Akt cleaved caspase-3 p-IκBα β-actin Abs

Actin Caspase 3 Cell cultures Iκbα Monoclonal antibody Mouse Protein Pten Rabbit Rock1

Top 5 similar protocols

Variable analysis

independent variables

Protein extraction from liver tissue or cell cultures

dependent variables

Notch1
P-JNK
ROCK1
P-Akt
Cleaved caspase-3
P-IκBα
β-actin

control variables

Monoclonal rabbit anti-mouse Notch1, NICD, Hes1, p-JNK, JNK, ROCK1, PTEN, p-Akt, Akt, TLR4, cleaved caspase-3, p-IκBα, and β-actin Abs (Cell Signaling Technology, MA)
Mouse monoclonal antibody JSAP1 (Santa Cruz Biotechnology)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!