Human normal thyroid (Nthy-ori3-1) cell lines and THCA cell lines (BCPAP, IHH4, 8505C, and BHT-10) were obtained from the American Type Culture Collection (ATCC) and then cultured in the medium-RPMI-1640 (Hyclone) together with 10% and 1% of FBS and penicillin-streptomycin (Sigma), respectively. Cells were preserved in 5% CO2 humidified incubator at 37°C [22 (link)].
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