Serum samples were collected at the time of sacrifice and stored at −80°C. Serum levels of Pro-collagen type 1 N terminal propeptide (P1NP) were measured using the Rat/Mouse P1NP EIA kit (Immunodiagnostic Systems, Fountain Hills, AZ, USA) and serum C-terminal telopeptide of type I collagen (CTX-1) levels were measured using RatLaps (CTX-1) EIA kit (Immunodiagnostic Systems Inc., Gaithersburg, MD, USA). The MILLIPLEX MAP mouse angiogenesis magnetic bead panel – multiplex assay was used to measure serum VEGF-A levels (MAGPMAG-24K, EMD Millipore, Billerica, MA, USA). All the assays were performed as recommended by the manufacturers and all the samples were assayed in duplicate. A standard curve was generated from each kit, and the absolute concentrations were extrapolated from the standard curves. The coefficients of variations for inter-assay and intra-assay measurements were < 10% for all assays and are similar to the manufacturers’ references [28 (link),29 (link)].
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Serum Biomarkers for Bone Turnover
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Angiogenesis
Assays
C terminal telopeptide of type i collagen
Collagen type 1
Immunodiagnostic
Mouse
P1np
Serum
Vegf
Corresponding Organization :
Other organizations : University of California, Davis
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Variable analysis
independent variables
- None explicitly mentioned
- Serum levels of Pro-collagen type 1 N terminal propeptide (P1NP)
- Serum C-terminal telopeptide of type I collagen (CTX-1) levels
- Serum VEGF-A levels
- Serum samples were collected at the time of sacrifice and stored at −80°C
- All the assays were performed as recommended by the manufacturers
- All the samples were assayed in duplicate
- A standard curve was generated from each kit, and the absolute concentrations were extrapolated from the standard curves
- The coefficients of variations for inter-assay and intra-assay measurements were < 10% for all assays and are similar to the manufacturers' references
- None explicitly mentioned
- None explicitly mentioned
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