Tissue, flash frozen in OCT at −80°C, was cryosectioned into 12- to 14-μm-thick sections, and sections were fixed with 4% paraformaldehyde as previously described (62 (link)). For examination of extracellular and membrane proteins, sections were preblocked in PBS containing 1% bovine serum albumin (BSA), 1:10 anti-mouse CD16/CD32 Fc block (eBioscience), and then stained with the appropriate antibodies in 1% BSA/PBS/Fc block for 2 hours at 4°C followed by fixation. To further visualize internal antigens, sections were then incubated overnight with the appropriate antibodies in PBS containing fish gelatin and saponin, followed by fixation (62 (link)). To identify nuclear localization of β-Cat, fixed sections were first permeabilized with 0.3% Triton X-100, followed by incubation with anti–β-Cat antibody (Abcam ab6302) in saponin block. All sections were mounted with Vectashield DAPI (4′,6-diamidino-2-phenylindole; Vector Laboratories, H-1200) and examined using a white light TSC SP8 Leica scanning confocal microscope. Sirius Red and fluorescence intensities (ΔCTCF) in threshold-matched images were determined using ImageJ.