Cytotoxicity of Magnolol in Cultured Neurons

According the method described previously [18] (link), cultured neurons were obtained from cerebral cortices of 1-day-old Sprague-Dawley rats. Cytotoxicity was determined at 24 hrs after treatment by using a LDH assay kit (Promega, Madison, WI) [18] (link), [24] (link), [25] (link). Experiments were undertaken on cultured neurons between 10 and 14 days in vitro (DIV). Neurons were incubated magnolol (0–300 µM) or vehicle (0.1% DMSO). The LD₅₀ value was defined as the concentration of compound required to induce 50% of cell deaths in 24 hrs at 37°C.

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Lee W.T., Lin M.H., Lee E.J., Hung Y.C., Tai S.H., Chen H.Y., Chen T.Y, & Wu T.S. (2012). Magnolol Reduces Glutamate-Induced Neuronal Excitotoxicity and Protects against Permanent Focal Cerebral Ischemia Up to 4 Hours. PLoS ONE, 7(7), e39952.

Publication 2012

After treatment Assay Cell deaths Cerebral cortices Cytotoxicity Dmso Magnolol Neurons Promega Sprague dawley rats

Corresponding Organization : Tzu Chi University

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Variable analysis

independent variables

Magnolol (0–300 µM)
Vehicle (0.1% DMSO)

dependent variables

Cytotoxicity at 24 hrs after treatment, determined using a LDH assay kit

control variables

Cultured neurons between 10 and 14 days in vitro (DIV)
Cultured neurons obtained from cerebral cortices of 1-day-old Sprague-Dawley rats

controls

Positive control: Not explicitly mentioned.
Negative control: Vehicle (0.1% DMSO)

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