Protocol detail

Mitochondrial Function Assessment in Mice

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Mice were killed 1 month after completion of the treatment cycles. XF24 Flux Analyzer (Agilent Technologies Inc, Santa Clara, CA) was used for measurement of mitochondrial oxygen consumption rate. Dorsal root ganglia neurons from 6 mice per group^{39 (link),42 (link)} or synaptosomes from 8 mice per group^{40 (link)} were isolated and tested as described previously.

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Ma J., Goodwani S., Acton P.J., Buggia-Prevot V., Kesler S.R., Jamal I., Mahant I.D., Liu Z., Mseeh F., Roth B.L., Chakraborty C., Peng B., Wu Q., Jiang Y., Le K., Soth M.J., Jones P., Kavelaars A., Ray W.J, & Heijnen C.J. (2021). Inhibition of dual leucine zipper kinase prevents chemotherapy-induced peripheral neuropathy and cognitive impairments. Pain, 162(10), 2599-2612.

Publication 2021

XF24 Flux Analyzer

Dorsal root ganglia Mice Mitochondrial Neurons Oxygen consumption Synaptosomes

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Variable analysis

independent variables

Treatment cycles

dependent variables

Mitochondrial oxygen consumption rate

control variables

Dorsal root ganglia neurons from 6 mice per group
Synaptosomes from 8 mice per group

controls

Positive control: Not specified
Negative control: Not specified

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