To evaluate the effect of Poly (I:C) on focal cerebral I/R injury, we employed a non-preconditioning regimen. Poly (I:C) (Catalog Cod: tlrl-picw, InvivoGen, San Diego, CA, USA) was dissolved in sterile endotoxin-free 0.9% NaCl and injected intraperitoneally (i.p., 10 μg/25 g bodyweight, n + 8) 1 hr prior to cerebral ischaemia (60 min.) followed by reperfusion for 24 hrs.
To investigate the therapeutic effect of Poly (I:C) on focal cerebral I/R injury, Poly (I:C) (i.p., 10 μg/25 g bodyweight, n + 8) was administered by intravascular injection 30 min. after the beginning of cerebral ischaemia. Focal cerebral ischaemia was continued for an additional 30 min. followed by reperfusion for 24 hrs.
To examine the role of TLR3 in Poly (I:C)-induced protection against cerebral I/R injury, TLR3 KO mice (n + 7/group) were treated with or without Poly (I:C) (10 μg/25 g bodyweight) 1 hr before the mice were subjected to focal cerebral ischaemia (60 min.) followed by reperfusion (24 hrs). The infarct size for all experiments was determined by triphenyltetrazolium chloride (TTC) staining as described below 9 (link),10 (link),13 (link),25 (link),26 (link).
To investigate the therapeutic effect of Poly (I:C) on focal cerebral I/R injury, Poly (I:C) (i.p., 10 μg/25 g bodyweight, n + 8) was administered by intravascular injection 30 min. after the beginning of cerebral ischaemia. Focal cerebral ischaemia was continued for an additional 30 min. followed by reperfusion for 24 hrs.
To examine the role of TLR3 in Poly (I:C)-induced protection against cerebral I/R injury, TLR3 KO mice (n + 7/group) were treated with or without Poly (I:C) (10 μg/25 g bodyweight) 1 hr before the mice were subjected to focal cerebral ischaemia (60 min.) followed by reperfusion (24 hrs). The infarct size for all experiments was determined by triphenyltetrazolium chloride (TTC) staining as described below 9 (link),10 (link),13 (link),25 (link),26 (link).
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