The 10,051 bp biotin-labeled dsDNA was generated by PCR39 (link) using the TaKaRa LA Taq kit (Takara Bio, Inc). The substrate DNA was lambda phage DNA (New England Biolabs) and the primers were as follows. “Lambda10kbForward”: (biotin TEG)-5′-CTGATGAGTTCGTGTCCGTACAACTGGCGTAATC and “Lambda10kbReverse”: 5′-ATACGCTGTATTCAGCAACACCGTCAGGAACACG (IDT DNA, Inc.).
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