Multivalent and multispecific receptors were constructed using the C-terminal SH3 domain of the human adaptor protein Gads and the synthetic protein Prb, as described previously23 (link). Multivalent and multispecific ligands incorporated the SH3 binding peptide (SBP) from the Gads cognate ligand SLP-7648 (link), as well as the synthetic designed Prbbinding DARPin, Pdar49 (link). Association and dissociation kinetics between ligand and receptor constructs were quantified by surface plasmon resonance measurements on a Biacore S200 instrument.
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Multivalent and Multispecific Receptor Construction
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Corresponding Organization : University of Minnesota
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- Multivalent and multispecific receptors constructed using the C-terminal SH3 domain of the human adaptor protein Gads and the synthetic protein Prb
- Multivalent and multispecific ligands incorporating the SH3 binding peptide (SBP) from the Gads cognate ligand SLP-76 and the synthetic designed Prb-binding DARPin, Pdar
- Association and dissociation kinetics between ligand and receptor constructs
- Surface plasmon resonance measurements on a Biacore S200 instrument
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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